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爱必信(上海)生物科技有限公司
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文献和实验Quantitative Measurement of the Concentration of Active Recombinant Retrovirus
Recombinant retroviruses are among several virus vectors currently being tested in clinical trials for purposes of gene therapy. As the number of clinical studies increases, accurate quantitation of retrovirus stocks and comparisons
One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin
The 6xHis/Ni-NTA system is a fast and versatile tool for the affinity purification of recombinant proteins and antigenic peptides. It is based on the high-affinity binding of six consecutive histidine residues (the 6xHis tag) to immobilized
/L of functional protein. Herein, we report on the production in Escherichia coli and the purification of a recombinant mature streptavidin bearing a T7-tag. Optimization of critical parameters, including the glucose concentration, the pH and the time
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