Recombinant Mouse IgG κ Protei

Laboratory-Scale Purification of a Recombinant E-Cadherin-IgG Fc Fusion Protein That Provides a Cell Surface Matrix for Extended Culture and Efficient

and growth factors derived from mouse Engelbreth–Holm–Swarm �sarcoma cells. The complexity and lot-to-lot variation of Matrigel preparations has prompted the search for more defined substrates that can support pluripotent stem cell culture. A recombinant

Purification of mAb (IgG)

(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10

ANTIBODY BINDING TO PROTEIN A AND PROTEIN G

A Protein G Human IgG1 ++++ ++++ Human IgG2 ++++ ++++ Human IgG3 - ++++ Human IgG4 ++++ ++++ Mouse IgG1 + ++++ Mouse IgG