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- 2025年07月13日
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文献和实验Assays for Pancreatic Triglyceride Lipase and Colipase
The assay of pancreatic triglyceride lipase (PL) is done routinely in clinical laboratories and in research laboratories with an interest in this lipolytic enzyme. The assay requirements differ between these two settings. The clinical
High-Level Baculoviral Expression of Lysosomal Acid Lipase
of the lipase such that structural and functional features of LAL have not been investigated to any great extent. Short-time expression systems generating recombinant enzyme in encaryotic cells, while suitable for studying effects of mutations of enzyme
Reticulocyte Lysate as a Model System to Study Endoplasmic Reticulum Membrane Protein Degradation
Recent studies have revealed that rabbit reticulocyte lysate (RRL) efficiently reconstitutes endoplasmic reticulum-associated degradation (ERAD) of mutant and misfolded membrane proteins. When supplemented with canine pancreas microsomal
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