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- 库存:
99
- 供应商:
爱必信(上海)生物科技有限公司
- 规格:
1mg
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文献和实验. It is very important to have equal concentrations of each dNTP (dATP, dCTP, dGTP, dTTP), as inaccuracy in the concentration of even single dNTP dramatically increases the misincorporation level. When maximum fidelity of the PCR process is crucial, the final dNTP
Strategy for accurate sequencing through hairpin structures
I; and adding increasing amounts of dGTP BigDye terminator (dGTP) chemistry to the standard BigDye v1.1 (BD) chemistry which contains dITP rather than dGTP. Sequencing results for each of the three DNA constructs are summarized in Table 2. Read
buffer 1 ul primer Heat 2 min. at 90~100℃ Add 1 ul of 1:4 dilution of s.s. binding protein. Leave 30 min. at room temperature. While waiting: Unfreeze label (35S or 32P dATP) Unfreeze Sequenase items Labelling mix (one for dGTP
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