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文献和实验Transposon Tagging with the En-l System
Transposon tagging (1 ) is a method for the isolation of genes, which display a mutant phenotype, Mutants that contain the transposon insert are “tagged” with the transposon and often display the characteristic chimeric or variegated
En Passant Mutagenesis: A Two Step Markerless Red Recombination System
Bacterial artificial chromosomes are used to maintain and modify large sequences of different origins in Escherichia coli . In addition to RecA-based shuttle mutagenesis, Red recombination is commonly used for sequence modification
Activation Tagging with En/Spm-I /dSpm Transposons in Arabidopsis
Activation tagging is a powerful strategy to find new gene functions, especially from genes that are redundant or show lethal knock-out phenotypes. It has been applied using T-DNA or transposons. En /Spm-I /dSpm engineered transposons
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