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- 爱必信(Absin)
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- abs826406
- 2025年07月06日
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文献和实验This chapter introduces a new method of maintaining human embryonic stem cells (hESCs) in the undifferentiated state through treatment with a GSK-3 inhibitor, BIO, under a feeder-free condition. Additionally, methods are introduced that determine
to an increase of β-catenin expression easily detectable by Westernanalysis. To find out the optimal concentration of GSK-3 inhibitor in cells of interest at certain time point, cells are treatedwith different concentration of drug (e.g., AR-A014418 as presented
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
buffer: 50 mM Tris-HCl, pH 7.5, 1% (v/v) TritonX-100, 0.27 M sucrose, 1 mM EDTA, 0.1 mM EGTA, 1 mMsodium orthovanadate, 50 mM sodium fluoride, 5 mM sodiumpyrophosphate, 0.1% (v/v) b-mercaptoethanol, and completeprotease inhibitor cocktail (Roche, Basel
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