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- 爱必信(Absin)
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- abs824730
- 2025年07月12日
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99
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爱必信（上海）生物科技有限公司
- 规格：
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文献和实验Purification and Characterization of Crustacean Calpain-like Proteinases
and calpeptin), iodoacetate, iodoacetamide, Hg2+ , pCMB. and JV-ethylmaleimide (NEM) (1,2 ,7 ). The serine proteinase inhibitors tosyllysyl chloromethyl ketone (TLCK), tosylphenylalanine chloromethyl ketone (TPCK), leupeptin, and antipain are effective
) (tosylphenylalanine chloromethyl ketone) TO MAKE 10 ml: Dissolve 50mg TPCK in DMSO to equal a final volume of 10 ml. * Note: The EDTA in the SUME buffer is also a protease inhibitor. Rapid Protein Prep Horvath and Riezman, Yeast , 1994 Stuff
Factor Xa Cleavage of MBP-Fusion protein
or affinity with pAminoBenzamidine - Agarose. Inactivation with 2µM dansyl-Glu-Gly-Arg-chloromethyl ketone (CALBIOCHEM #251700) or with 1mM PMSF. Cleavage conditions according to QUIAGEN Buffers: recommend 20mM Tris HCl pH6.5; 50mM NaCl and 1mM CaCl
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