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文献和实验SDS Gel Electrophoresis of Tubulin\MAPs
% TCA Procedure Prepare 15 cm glass tubes with 6 mm internal diameter. Polymerize a 10 cm separation gel and a 1 cm stacking gel within the tubes by the addition of TEMED and ammonium persulfate as directed in Chapter Four.
, which should slowly drop off. Watch the above, you have been warned!Gel Electrophoresis. (a) IEF strips. IPG strips are plastic backed, come in various lengths, but are usually 3.0mm wide and about 0.5mm thick when rehydrated. They come, dehydrated, in a variety
from the bottom of the cassette. 3) In one smooth motion, gently pull the comb out of the cassette. 4) Rinse the sample wells with the appropriate 1X SDS Running Buffer. Invert the gel and shake the gel to remove the buffer. Repeat two more times.
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