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- 库存:
99
- 供应商:
爱必信(上海)生物科技有限公司
- 规格:
50ug
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文献和实验The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags
is unwanted or may represent a disadvantage for the projected use of the protein, like in clinical, functional or structural studies. For N-terminal tags, the TAGZyme system represents an ideal approach for fast and accurate tag removal. TAGZyme is based
, and usually final protein yield between 0.1 and 2 mg/g cells is acceptable. Another concern is protein degradation. Especially with Pichia , protease activity during cell lysis and purification is always an issue. The importance of N-terminal degradation
Isolation and Enzymatic Activity of Angiogenin
Angiogenin is one of several unusual members of the pancreatic ribonuclease superfamily (for reviews see refs. 1 ,2 ). It was first isolated as a 14 kDa soluble protein from culture medium conditioned by human colon carcinoma (HT-29) cells
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