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- 库存:
99
- 供应商:
爱必信(上海)生物科技有限公司
- 规格:
1g
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文献和实验James Hardwick CNBr Cleavage Procedure
tube containing 200 microliters of 100mg/ml CNBr in 70% formic acid. CNBr stocks are 200 milligrams/ml in 70% formic acid and are stored at -70°C. 70% formic acid is made from 98-100% formic acid and MQH2O. Optional: If you want to know
6.8),5ml 50%甘油,2ml 10%的SDS,0.5ml巯基乙醇,1ml 1%溴酚蓝,0.9ml蒸馏水。可在4℃保存数周,或在-20℃保存数月。2. 凝胶贮液:在通风橱中,称取丙烯酰胺30g,甲叉双丙烯酰胺0.8g,加重蒸水溶解后,定容到100ml。过滤后置棕色瓶中,4℃保存,一般可放置1个月。3. pH8.9分离胶缓冲液: Tris 36.3g ,加1mol/L HCl 48ml,加重蒸水80ml使其溶解,调pH8.9,定容至100ml, 4℃保存。4. pH6.7浓缩胶缓冲液: Tris 5.98
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELS
WARNING: wear gloves 100 ml solution 18.98 g acrylamide 1.02 g BAC (N',N'-bis-acrylylcystamine) Heat approximately 75 ml of water to almost boiling. Add acrylamide and cover with a watch glass. Mix briefly to dissolve and reheat
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