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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
爱必信(上海)生物科技有限公司
- 规格:
500mg
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文献和实验from described in our paper. 1) 50°C 2 min, 1 cycle 2) 95°C 10 min, 1 cycle 3) 95 °C 15 s -> 60 °C 30 s -> 72 °C 30 s, 40 cycles 4) 72°C 10 min, 1 cycle 3. A real-time PCR reaction mixture can be either 50 ml or 25 ml. Prepare the following
may occur. The primer should not be self-complementary or complementary to any other primer in the reaction mixture, in order to avoid primer-dimer and hairpin formation. The melting temperature of flanking primers should not differ by more than 5°C
【转载】protocol for real-time PCR
, 1 cycle 2) 95°C 10 min, 1 cycle 3) 95 °C 15 s -> 60 °C 30 s -> 72 °C 30 s, 40 cycles 4) 72°C 10 min, 1 cycle 3. A real-time PCR reaction mixture can be either 50 ml or 25 ml. Prepare the following mixture in each optical tube. 25 ml SYBR Green
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