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- 2025年07月09日
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文献和实验Gene Knockout Techniques(1) The Knockout Process
50 (three spots of worm suspension per plate).Synchronize Day 3 (Friday) Synchronize population by egg prep, following the procedure in Barstead, R.J. 1999. Reverse Genetics in ?C. elegans. A Practical Approach.? I.A. Hope, editor, Oxford University Press, NY
Mutational Analysis of the Human Protein C Gene
The single gene for protein C is located at position q13–q14 on chromosome 2 (1 ). Two groups have described human genomic clones of protein C isolated from phage l charon libraries using cDNA for human protein C as hybridization probes
volume of 35 ml at room temperature. 2) Add the diluted buffy coat on top of 15 ml of Lymphoprep. 3) Centrifuge at 160 x g for 20 minutes at 20°C. Allow to decellerate without brakes. 4) Remove 20 ml of supernatant
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