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文献和实验Gene Knockout Techniques(1) The Knockout Process
to save time and materials, but everything can be done in 96-well plates if you don?t have access to 384-well equipment. 10 ul reactions work fine in 96-well plates, and don?t require nearly as much Taq. To date we have done most steps with worms grown on solid
以最火热的 p53 为例Step1 先找 p53 基因序列1. NCBI--输入 p53 并选择物种 human：tumor protein p53 [Homo sapiens (human)]https://www.ncbi.nlm.nih.gov/gene2. Ctrl+F，输入 sequence，快速定位（当然其实拉下去就是了），点击 Genebank3. 下载 p53 基因序列4. 用 SnapGene 软件打开序列，并简单了解每个元件最好是可以学一下 SnapGene 这个软件的使用
Gene Knockout In Murine Embryonic Stem (ES) Cells
from the PMEFs as this is a differentiating agent). Also check ES cells, at this point they should be a nice dense monolayer, try to get a feel for the numbers, don't want too many cells (with time doing this it should be possible to have an accurate number
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