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- 爱必信(Absin)
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- abs6103651
- 2025年07月16日
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文献和实验Gene Knockout Techniques(1) The Knockout Process
and materials lists. If you have questions about anything that we have not covered, please be sure to contact us.Library Construction We use our own subculture of the wild type (N2) to make mutagenized libraries. To minimize accumulation of spontaneous mutations
us to change genomic sequences at will to control the fundamental geneticinformation within a cell. It has been implemented in creatingmodel cell lines or organisms for the study of gene functions orgenetics of human diseases, and in gene therapy
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
3) Day 1: Replace medium and culture cells a. 24 hours after transduction, replace the medium with fresh fibroblast medium. b. Note: Depending on your cell type, you should expect to see some cytotoxicity 24–48 hours post
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