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- 2025年07月13日
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爱必信（上海）生物科技有限公司
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文献和实验Protocol for intracytoplasmic staining of cytokines for FACS analysis
S-2149). 7) We suggest the following antibody conjugates (usually at 10mcg/ml or as manufacturers recommendations) to detect mouse cytokines, added in saponin buffer for 30 mins at 4 deg C. I have grouped them in compatible pairs for multicolour
Diego, CA); GSK-3α (Upstate, LakePlacid, NY); and b-actin (Sigma, St. Louis, MO). 7. Secondary antibodies: anti-mouse and anti-rabbit IgG conjugatedto horseradish peroxidase (Santa Cruz Biotechnology,Santa Cruz, CA). 8. Enhanced chemiluminescent
Intracellular Cytokine Staining Protocol
)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinJES6-5H4IL-4mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinBVD6-24G2IL-6mouse
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