PE Rat IgG2b Isotype Control

Immunofluorescent Staining of Mouse and Rat Leukocytes

secondary reagent (e.g., fluorochrome-conjugated avidin, streptavidin, anti-Ig allotype, anti-Ig isotype, polyclonal anti-Ig). For example, dilute antibody to ~1 µg per 100 µl in wash buffer and add this to each well containing the loosened cell pellet

Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas

7.16 anti-rat IgG2b). -------------------------------------------------------------------------------- 5) For full details see: Clark, M.R., & Waldmann, H. Production of murine monoclonal antibodies in Methods in Hematology 13:1-20 (1986

Purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion

D  Granulocytic : Gr-1 (Ly-6G) IgG2b, 01211A  D8 IgG2b, 09821D  Eryth: Ter-119 IgG2b, 09081A  Sca-1 PE-IgG2a (E31-161.7) 01835B  PE-IgG2a Isotype control, 11025