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- 爱必信(Absin)
- 中国
- abs1860002
- 2025年07月05日
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- 供应商：
爱必信（上海）生物科技有限公司
- 规格：
100T
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文献和实验Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
Immunofluorescent Staining of Mouse and Rat Leukocytes
16 (Mouse BD Fc Block™; Cat No. 553141/553142) or anti-rat CD32 (Rat BD Fc Block™; Cat. No. 550270/550271) is recommended. BD Fc Block™ can be added to cells (~0.25 µg per million cells, 3 - 5 min, 4°C) and need not be washed out prior to addition of primary
volume to bring the cells to the following concentrations: Type Cell Concentration mouse or rat 1.5 x 106 cells per ml hamster 0.5 - 1.0 x 106 cells per ml
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