- ￥2300
- 爱必信(Absin)
- 中国
- abs173157
- 2025年07月10日
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- 库存：
99
- 供应商：
爱必信（上海）生物科技有限公司
- 规格：
100uL
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文献和实验which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates
Polyclonal Antibody Production
Zealand White Rabbits and have them delivered to the ARC. Don’t put all your eggs in one basket only using one rabbit. Let them get accustomed to there home there for two weeks before the first pre-bleed. Pre-bleed
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
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