- 询价
- 辰辉创聚生物
- 中国
- NBAB-109543
- 2025年07月09日
- WB
- Human, Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus Predicted to React with: Phospho-mTOR (Ser2448) Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide corresponding to the residues around Ser2448 of Human Phospho-mTOR
- 亚型：
Monoclonal Rabbit IgG
- 保存条件：
This liquid antibody can be stored at 2℃-9425℃ for at least two weeks. Avoid repeated freeze/thaw cycles.
- 克隆性：
单克隆
- 适应物种：
Human, Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus Predicted to React with: Phospho-mTOR (Ser2448) Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus
- 保质期：
see COA
- 库存：
大量
- 供应商：
辰辉创聚生物
- 应用范围：
WB
- 靶点：
Phospho-mTOR (Ser2448)
- 抗体英文名：
Phospho-mTOR (Ser2448) antibody
- 抗体名：
Phospho-mTOR (Ser2448)抗体
- 规格：
100ug;500ug;1mg
| 产品信息
品名:NebuSelect™ Phospho-mTOR (Ser2448) Monoclonal Antibody
货号:NBAB-109543
品牌:Nebulabio
规格:100ug;500ug;1mg
| 产品描述
| 产品属性
Product Category：Rabbit MAb
Reactivity：Human, Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus Predicted to React with: Phospho-mTOR (Ser2448) Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus
Specificity：Human Phospho-mTOR (Ser2448) Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus
Immunogen：A synthetic peptide corresponding to the residues around Ser2448 of Human Phospho-mTOR
Isotype：Monoclonal Rabbit IgG
Purification：Protein A
Buffer：10 mM HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5
Conjugate：Unconjugated
Applications：WB
Dilution：WB: 1:2000-1:20000
Storage：Store at 4°C for short-term, -20°C for long-term. Avoid repeated freezing and thawing cycles.
For Research Use Only!
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文献和实验Evaluation of the Nutrient-Sensing mTOR Pathway
mTOR, an evolutionarily conserved Ser/Thr protein kinase, belongs to the PI3K-related kinase family, which also includes DNA-PKcs, ATM, and ATR. Although other PI3K-related kinase family members have been shown to secure genomic integrity
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
目的：通过特异性阻断PI3K和mTOR，观察HepG2和Hep3B细胞株PI3K/Akt/mTOR信号通路活性及生物学行为的改变，探讨相关的分子机制。 方法：在培养的HepG2、Hep3B人肝癌细胞株和人正常肝细胞株QSG-7701上，以免疫印迹方法（Western blot）检测各细胞株中PI3K（p110α亚单位）、PTEN、pAkt（S473，T308）和p-mTOR（S2448）的表达情况；分别用 PI3K抑制剂LY294002（50μmol/ml）和mTOR抑制
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