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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic peptide corresponding to residues around Ser9 of Human Phospho-GSK3 beta
- 亚型:
Monoclonal Rabbit IgG
- 保存条件:
This liquid antibody can be stored at 2℃-6160℃ for at least two weeks. Avoid repeated freeze/thaw cycles.
- 克隆性:
单克隆
- 适应物种:
Human, Species predicted to react based on 100% sequence homology: mouse, rat
- 保质期:
see COA
- 库存:
大量
- 供应商:
辰辉创聚生物
- 应用范围:
WB
- 靶点:
Phospho-GSK3 beta (Ser9)
- 抗体英文名:
Phospho-GSK3 beta (Ser9) antibody
- 抗体名:
Phospho-GSK3 beta (Ser9)抗体
- 规格:
100ug;500ug;1mg

| 产品信息
品名:NebuSelect™ Phospho-GSK3 beta (Ser9) Monoclonal Antibody
货号:NBAB-106278
品牌:Nebulabio
规格:100ug;500ug;1mg
| 产品描述
| 产品属性
Product Category:Rabbit MAb
Reactivity:Human, Species predicted to react based on 100% sequence homology: mouse, rat
Specificity:Human Species predicted to react based on 100% sequence homology: mouse, rat
Immunogen:A synthetic peptide corresponding to residues around Ser9 of Human Phospho-GSK3 beta
Isotype:Monoclonal Rabbit IgG
Purification:Protein A
Buffer:0.2 μm filtered solution in PBS
Conjugate:Unconjugated
Applications:WB
Dilution:WB: 1:1000-1:5000
Storage:Store at 4°C for short-term, -20°C for long-term. Avoid repeated freezing and thawing cycles.
For Research Use Only!
To get more information, please contact us freely.
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文献和实验下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
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