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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
3'-Sialyllactose (3'-SL) / GM3 Ganglioside oligosaccharide with free terminal amine (Linker-NH2 C) Sodium salt
- 库存:
100+
- 供应商:
上海维亦特生物科技有限公司
分子量:876.84
性状:White powder
储存:室温
溶解性:in water, PBS or HEPES
来源:Synthesis
附件:MS
糖链结构:Neu5Acα2-3Galβ1-4Glc-NAc-CH2-(1.4-Tz)-(CH2)2-EG7-NH2
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis
Cell cycle analysis of Escherichia coli cells
forks → 8-origin peak in run-out histogram = 77% of the cells 6 and 4 forks → 4-origin peak in run-out histogram = 23% of the cells The fraction of cells containing 6 forks: F = 2 - 2((τ-at )/τ) = 2 � 2((27-2)/27) = 0.10 The fraction of cells
Single Primer ("Semi-Random") P
Po: F1284 (TP89) 38 bp from end; will not work in TPOPs; CAAGATGTGTATCTACCTTAAC Po or Pn: IS10R2 (TP134) 27 bp from end; right side of TPOPs only; CAAGATGTGTATCCACCTTAACTTAATGATTTT Pn: IS10R4 (TP134) 4 bp from end; any Tn10 derivative
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