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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic phosphopeptide corresponding to residues around Tyr1173 of the Human Phospho-EGF Receptor
- 亚型:
Monoclonal Rabbit IgG
- 保存条件:
This liquid antibody can be stored at 2℃-4618℃ for at least two weeks. Avoid repeated freeze/thaw cycles.
- 克隆性:
单克隆
- 适应物种:
Human, Species predicted to react based on 100% sequence homology: cynomolgus
- 保质期:
see COA
- 库存:
大量
- 供应商:
辰辉创聚生物
- 应用范围:
WB
- 靶点:
Phospho-EGF Receptor (Tyr1173)
- 抗体英文名:
Phospho-EGF Receptor (Tyr1173) antibody
- 抗体名:
Phospho-EGF Receptor (Tyr1173)抗体
- 规格:
100ug;500ug;1mg

| 产品信息
品名:NebuSelect™ Phospho-EGF Receptor (Tyr1173) Monoclonal Antibody
货号:NBAB-104736
品牌:Nebulabio
规格:100ug;500ug;1mg
| 产品描述
| 产品属性
Product Category:Rabbit MAb
Reactivity:Human, Species predicted to react based on 100% sequence homology: cynomolgus
Specificity:Human Species predicted to react based on 100% sequence homology: cynomolgus
Immunogen:A synthetic phosphopeptide corresponding to residues around Tyr1173 of the Human Phospho-EGF Receptor
Isotype:Monoclonal Rabbit IgG
Purification:Protein A
Buffer:0.2 μm filtered solution in PBS
Conjugate:Unconjugated
Applications:WB
Dilution:WB: 1:5000-1:50000
Storage:Store at 4°C for short-term, -20°C for long-term. Avoid repeated freezing and thawing cycles.
For Research Use Only!
To get more information, please contact us freely.
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文献和实验运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
results of phosphorylated proteins磷酸化的试剂盒中的抗体是经过大量筛选,选取了高特异性和低背景的抗体。p-P38MAPK抗体是基于P38的磷酸化Thr180到Tyr182这个位点多肽片段所制备。P-JNK抗体是基于人的Thr183和Tyr185.这个抗体可以同时识别P-JNK1和P-JNK2.我们在运用Cell Based Elisa P38和JNK两个试剂盒做检测的同时,又用传统的WB方式来验证这个结果。经过反复验证,都得出了同样的结果, Cell Based
signaling公司的Phospho-PI3K p85 (Tyr458)/p55(Tyr199) 我自己分析没有条带无非是细胞状态不好,或者抗体不好,不知道还有没有可能是没有加磷酸化酶抑制剂,以至于磷酸化的蛋白被去磷酸化了? 谢谢啦~ yhwangcams 检测内参蛋白如GAPDH,ACTIN,TUBULIN是否成功? 用PLB裂解细胞得到的蛋白适合做REPORTER,做WESTERN不太合适。建议可以用其他BUFFER裂解蛋白。
【翻译】Development trends for monoclonal antibody cancer therapeutics
; ImClone/Bristol-Myers Squibb), nimotuzumab (TheraCIM; YM BioSciences) and panitumumab (Vectibix; Amgen). These products inhibit binding of EGF and subsequent activation of the EGF receptor33, 34. The in vivo mechanism of action of trastuzumab
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