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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海淳麦生物
- 适应物种:
人/大鼠/小鼠等各类物种
- 样本:
血清 细胞等
- 规格:
48T/96T
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
2. Table top centrifuge capable at least 13,000 x g 3. 1.5 or 2 ml Nuclease-Free centrifuge tubes 4. 0.45 μm filter unit 实验步骤 Before starting: 1. Fresh or flash-frozen cultured cells can be used
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