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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
冷冻-20℃
- 保质期:
咨询
- 英文名:
N4-Acetyl-2'-deoxy-5'-O-DMT-cytidine 3'-Sp-oxazaphospholidine
- 库存:
100+
- 供应商:
上海维亦特生物科技有限公司
N4-Acetyl-2'-deoxy-5'-O-DMT-cytidine 3'-Sp-oxazaphospholidine
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文献和实验Inverse PCR & Cycle Sequencing
2.0ul ddH2 O 8.0 or 9.5ul Sau3A I, HinP1 I, or Msp I 10 units 1) Incubate 2.5hrs at 37°. 2) Heat for 20min to 65°. 3) [Optional: Check 10ul of each digest
Establishment of Stable Transfectant of CHO Lec Cells
medium: To 9 lt of mili-Q water, add MEM w/o Gln (GIBCO, #11700-077). Pwdr for 10 lt.NaHCO3 , 27.75gglucose: 30 gnucleosides: adenosine (Sigma A-4036), 70mg; guanosine (Sigma G-6264), 70mg; cytidine (Sigma C-4654), 70mg; uridine (Sigma U
Establishment of Stable Transfectant of CHO Lec Cells
) by yourself but this cannot be used as serum-free medium. Recipe for 10 lt of J/J medium: To 9 lt of mili-Q water, add MEM w/o Gln (GIBCO, #11700-077). Pwdr for 10 lt. NaHCO3 , 27.75g glucose: 30 g nucleosides: adenosine (Sigma A-4036), 70
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