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文献和实验In Vitro Digestion with Proteases Producing MHC Class II Ligands
digestion assays of protein with recombinant proteases or purified endosomes/lysosomes, T cell stimulation) to monitor the production of MHC class II ligands.
Studying MHC Class II Peptide Loading and Editing In Vitro
of major histocompatibility complex (MHC) class II molecules upon binding of antigenic peptides and the effect of DM on the interactions. Using purified soluble molecules, in this chapter, we describe several in vitro methods for measuring peptide binding to HLA-DR molecules
Detection of Class Switching and IgE Synthesis in Human B Cells
events leading to IgE synthesis. This chapter will illustrate how human B cells (na�ve or memory) can be purified, stored, and cultivated in medium that is permissive for IgE synthesis and stimulated with IL-4 or IL-13 and CD40 activation
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