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- 文献和实验
- 技术资料
- 保存条件:
冷冻-20℃
- 保质期:
咨询
- 英文名:
Vasopressin Dimer (anti-parallel) (TFA)
- 库存:
100+
- 供应商:
上海维亦特生物科技有限公司
分子量:2282.49
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文献和实验prior to rehydration with enzyme.After digestion,the peptides are extracted from the gel and mass analyzed.In-gel digestion procedureMaterials25 mM NH4HCO3 in 50% acetonitrile25 mM NH4HCO3,pH 85% TFA/ 50% acetonitrile0.1 mg/ml trypsin (sequence grade,Promega)in 25 mM
.These are only rough guidelines. Detailed instructions about PCR laboratory setup and maintenance may be found in PCR Methods and Applications, 3, 2, S1-S14, 1993.Preparation of Reaction Mixture To perform several parallel reactions, we recommend the preparation
and negative control be conducted in parallel to all analyses. The positive control can be a marker or known protein while the negative control should be a blank region of the gel. Wash twice with 50µL of 18 Mohm water for approx 15 minutes. Wash
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