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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
冷冻-20℃
- 保质期：
咨询
- 英文名：
Kaur-16-en-18-oic acid, 15-oxo- (7CI,8CI)
- 库存：
100+
- 供应商：
上海维亦特生物科技有限公司
- CAS号：
6620-00-4
分子量：316.441
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文献和实验with ITS (4 ml/L should yield 5 mg/L insulin and 5 mg/L transferrin). --> "Appropriate" = bring cells to a final concentration of 5 X 105 cells/ml. 4) Add [3H] arachidonic acid to get ~2 µCi/ml (2 X 106 µCi/ml???). --> Make sure final AA
Measurement of -Carotene15,15-Dioxygenase Activity by Reverse-Phase HPLC
; however, the majority of β-carotene (60–70%) is metabolized to retinyl esters and transported by the lymph (1 ). Indeed, in the enterocyte of the small intestine, β-carotene 15,15′-dioxygenase (EC 1.13.11.21), acytosolic enzyme, cleaves the central double bond (15-15′
] arachidonic acid to get ~2 µCi/ml (2 X 106 µCi/ml???). --> Make sure final AA concentration is in the nM range. 5) Incubate cells with label for a period of 60 minutes (2 hours???). 6) Remove label by washing cells 3 X with ice cold media
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