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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Short term 4℃; Long term -20℃.
- 保质期:
见COA
- 英文名:
Recombinant Human Azurocidin/CAP37/AZU1/HBP, His-tag
- 库存:
大量
- 供应商:
北京辰辉创聚生物技术有限公司
- 规格:
100ug;1mg;Bulk

| 产品信息
品名:NebuSelect™ Recombinant Human Azurocidin/CAP37/AZU1/HBP, His-tag
货号:NBL-242552
品牌:Nebulabio
规格:100ug;1mg;Bulk
| 产品描述
NebuSelect™ Recombinant Human Azurocidin/CAP37/AZU1/HBP, His-tag(Cat#NBL-242552) is expressed in HEK293 with His tag at the C-Terminus.It contains Ile27-Pro250.
| 产品属性
表达系统:HEK293
标签:His-tag
靶点名称:Azurocidin/CAP37/AZU1/HBP
物种:Human
分子量:The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Bis-Tris PAGE result.
氨基酸序列:Ile27-Pro250
Accession: P20160
纯度:>95%
内毒素:<1EU/ug(LAL Method)
储存运输条件: Short term 4℃; Long term -20℃.
| 靶点信息
Heparin-binding protein (HBP), also known as cationic antimicrobial protein 37 (CAP37) and Azurocidin, is a member of the serine protease family that includes Cathepsin G, neutrophil elastase (NE), and proteinase 3 (PR3). This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope.
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文献和实验下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
扫描仪中,都采用机械式的二维X,Y线性扫描技术实现,即X,Y方向都采用直线驱动器和直线导轨实现往复运动。此类装置,由于驱动系统的频率限制,驱动器的扫描惯性大,使得扫描效率低,分析时间相当长;并且往复行程长,对直线导轨的精度要求相当高。二、光机结合的二维扫描系统为同样实现生物芯片的二维扫描,我们的实验装置设计如图2,采用了振镜和大数值孔径的远心f-è物镜相结合实现X方向扫描,Y方向的运动仍采用直线驱动器和直线导轨实现。 系统中,对于f-è物镜,满足x=2fè(è为振镜的摆动角度,f为物镜焦距)的线性
Analysis of RB Action in DNA Damage Checkpoint Response
checkpoint response: (1) transcriptional repression of E2F-regulated genes (cyclin A reporter assay); (2) induction of cell cycle arrest (Brd-U incorporation assay); and (3) inhibition of DNA double-strand break accumulation (phosphorylated-histone H2A.X
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