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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Short term 4℃; Long term -20℃.
- 保质期:
见COA
- 英文名:
Recombinant Mouse IL-20, hFc-tag
- 库存:
大量
- 供应商:
北京辰辉创聚生物技术有限公司
- 规格:
100ug;1mg;Bulk

| 产品信息
品名:NebuSelect™ Recombinant Mouse IL-20, hFc-tag
货号:NBL-242485
品牌:Nebulabio
规格:100ug;1mg;Bulk
| 产品描述
NebuSelect™ Recombinant Mouse IL-20, hFc-tag(Cat#NBL-242485) is expressed in HEK293 with hFc tag at the N-Terminus.It contains Leu25- Leu176.
| 产品属性
表达系统:HEK293
标签:hFc-tag
靶点名称:IL-20
物种:Mouse
分子量:The protein has a predicted MW of 44.9 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.
氨基酸序列:Leu25-Leu176
Accession: Q9JKV9
纯度:>95%
内毒素:<1EU/ug(LAL Method)
储存运输条件: Short term 4℃; Long term -20℃.
| 靶点信息
Interleukin (IL)-20 is a member of the IL-10 family of cytokines, which has been reported to participate in autoimmune inflammatory diseases. However, the potential role of IL-20 in hepatocellular carcinoma (HCC) progression has not yet been investigated. In addition, IL-20 expression was significantly associated with tumor size, metastasis, TNM stage and poor prognosis in patients with HCC.
For Research Use Only!
To get more information, please contact us freely.
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文献和实验, and genotype for each mouse that is tagged and tailed. Keep the log sheets with the pedigree. 4. Together, the pedigree, mating cards, and log sheets should provide the following information for each mouse: birth date, sex, ear tag number, coat color, DNA
降低洗脱条件的严格程度。变性条件下纯化目的蛋白 缓冲液条件不正确 ——检查漂洗缓冲液的 pH 值和组成确保体系中不含鳌合剂及还原剂 2、蛋白在纯化过程中形成沉淀 温度过低 ——改为室温纯化 蛋白形成聚合物 ——尝试加入增加溶解度的试剂,如0.1% Triton×-100 或Tween-20,高达10mM β-ME 或1mM THP,高达2MNaCI 或帮助稳定蛋白的因子,如 Mg2+。某些情况下,可能需要
使它们得以能进行纯化但这也意味着需要对每一种待纯化的蛋白质研发一套新的方法。所幸的是,尽管存在这种固有的困难,但现已有多种方法可以利用,蛋白质纯化策略也已实际可行。目前,待研究蛋白或酶的基因的获得已是相当普遍的事。可诱导表达系统特别是Studier等发展的以噬菌体T7RNA聚合酶为基础的表达系统的出现使人们能近乎常规地获得过表达(overexpression),表达水平可达细胞蛋白的2%以上,有些甚至高达50%.(一)试剂准备采用T7· Tag Affinity Purification Kit1. T
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