- ￥2900
- AntibodySystem
- France
- RHH20009
- 2025年07月10日
- WB
- Rabbit
- Human, Mouse, Rat, Hamster
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
- 克隆性：
Monoclonal
- 适应物种：
Human, Mouse, Rat, Hamster
- 保质期：
1 year
- 级别：
科研级
- 库存：
99999
- 供应商：
AntibodySystem
- 宿主：
Rabbit
- 应用范围：
WB
- 浓度：
1 mg/ml
- 靶点：
Mothers against decapentaplegic homolog 2, SMAD 2, SMAD2, JV18-1, Mad-related protein 2, hMAD-2, MADR2, Mothers against DPP homolog 2, SMAD family member 2, MADH2, Smad2, hSMAD2, MAD homolog 2
- 抗体英文名：
Anti-Phospho-SMAD2 (Ser250) Antibody (R2G68)
- 抗体名：
Anti-Phospho-SMAD2 (Ser250) Antibody (R2G68)
- 规格：
100 μg
| 克隆性 | Monoclonal |
| 宿主 | Rabbit |
| 同种型 | IgG |
| 种属反应性 | Human, Mouse, Rat, Hamster |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide. |
| 浓度 | 1 mg/ml |
| 纯度 | >95% by SDS-PAGE. |
| 应用 | WB |
| 靶标 | Mothers against decapentaplegic homolog 2, SMAD 2, SMAD2, JV18-1, Mad-related protein 2, hMAD-2, MADR2, Mothers against DPP homolog 2, SMAD family member 2, MADH2, Smad2, hSMAD2, MAD homolog 2 |
| 纯化 | Protein A/G purified from cell culture supernatant. |
| 内毒水平 | Please contact with the lab for this information. |
| Accession号 | Q15796 |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt. |
| 克隆号 | R2G68 |
| 备注 | For research use only. |
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文献和实验水研磨成匀浆，转移到 离心管 中，再用6mL 蒸馏水分次洗涤研钵，洗涤液收集于同一 离心管 中，放置0.5~1h 以充分提取，然后在4 000r/min 离心20min，弃去沉淀，上清液转入10mL 容量瓶，并以蒸馏水定容至刻度，即得待测样品提取液。 （2）测定：另取2 支10mL 具塞 试管 ，按下表取样。吸取提取液0.1mL（做一重复），放入具塞刻度 试管 中，加入5mL 考马斯亮蓝G-250 蛋白
Monoclonal Antibody Production
) (3) 250 ml bottles of 100X penicillin-streptomycin (4) 10 ml of 100X HAT selection solution (5) 10 ml of 100X HT solution (6) 10 sterile flat-bottomed 96-well plates 3. Assaying for Positive Clones Run an ELISA.html">indirect ELISA using
Polyclonal Antibody Production
from the muscle, insert the needle 1-2 cm at a shallow angle so as to not hit muscle, and inject 250 µL of your innoculum. After the required volume has been injected, let the needle sit for a few seclonds, then pull out and gently rub the injection site
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