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- 英文名:
Ethyl 4-(8-chloro-2-(2,3,4,5,6-pentahydroxyhexanoyl)-5H-benzo[5,6]cyclohepta[1,2-b]pyridin-11(6H)-ylidene)piperidine-1-carboxylate
- 库存:
100+
- 供应商:
上海维亦特生物
- CAS号:
N/A
Ethyl 4-(8-chloro-2-(2,3,4,5,6-pentahydroxyhexanoyl)-5H-benzo[5,6]cyclohepta[1,2-b]pyridin-11(6H)-ylidene)piperidine-1-carboxylate
N/A
C₂₈H₃₃ClN₂O₈
561.02
No Data Available
No Data Available
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文献和实验Ethyl 4-(8-chloro-2-(2,3,4,5,6-pentahydroxyhexanoyl)-5H-benzo[5,6]cyclohepta[1,2-b]pyridin-11(6H)-ylidene)piperidine-1-carboxylate
N/A
C₂₈H₃₃ClN₂O₈
561.02
No Data Available
No Data Available
of samples). 13. Add 0.5 ml Ethyl Acetate to each tube. 14. Vortex 10" each tube & then mix by inverting for another 30". 15. Spin @ 14K RPM for 30"-1'. 16. Remove 450 µl of organic (upper) phase. 17. Speed vac for 15'. 18. Resuspend in 30 µ
). Some investigators use a 0.25% solution of Coomasie Blue in methanol-water-glacial acetic acid (5-5-1). Copper sulfate (CuSO 5H O MW 249.68) 0.5% (w/v) Dissolve 0.13 grams of Alcian Blue 8GX (Sigma
Phycoerythrin conjugation protocol
(Molecular Probes) DTT Dithiothreitol NEM N-ethyl maleimide * 10 ml Sephadex G-10 columns (2) Separation column A long Sephacryl S-300 column
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