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- 英文名:
O-Tri-t-Butyldimethylsilyl Tylosin Acetate
- 库存:
100+
- 供应商:
上海维亦特生物
- CAS号:
N/A
O-Tri-t-Butyldimethylsilyl Tylosin Acetate
N/A
C₆₅H₁₁₉NO₁₈Si₃
1286.89
No Data Available
No Data Available
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文献和实验O-Tri-t-Butyldimethylsilyl Tylosin Acetate
N/A
C₆₅H₁₁₉NO₁₈Si₃
1286.89
No Data Available
No Data Available
Replication timing by density transfer
. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289
Replication timing by comparative hybridization
of minimal medium, just to avoid having the dry Pronase stick to the sides of the flask.] 6. Hold at 37o C for 90-120 min (until budded cells accumulate). Remove one sample (t = 0), then swirl the flask in ice-water and return it to 23o C. Continue
Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
Acetate gamma-[32P]-ATP T4 Polynucleotide Kinase Sodium Acetate Ammonium Acetate Ethanol DTT Restriction Enzyme Tris Zinc Chloride Spermidine Restriction Enzyme Buffer Glycogen BSA
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