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- 英文名：
5-Azaspiro[2.4]heptane Trifluroacetate
- 库存：
100+
- 供应商：
上海维亦特生物
- CAS号：
1420271-30-2
5-Azaspiro[2.4]heptane Trifluroacetate
1420271-30-2
C₆H₁₁N • C ₂HF₃O₂
N/A
No Data Available
No Data Available
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文献和实验5-Azaspiro[2.4]heptane Trifluroacetate
1420271-30-2
C₆H₁₁N • C ₂HF₃O₂
N/A
No Data Available
No Data Available
and dip into a scintillation vial containing 5ml PBS/5ml heptane. The embryos should slide off. Those with vitelline membranes intact will stay at the interphase, while hatched larvae will settle to the bottom. Use a paint brush to remove remaining larvae
in 3% bleach until embryos float to the surface (1-5 min). Don't worry about over-dechorionating. Rinse with water. -remove screen and dip into a scintillation vial containing 5ml PBS/5ml heptane. The embryos should slide off. Those with vitelline membranes
RNA in situ protocol for DNA and RNA probes
Other reagents needed: Drosophila saline (0.7% NaCl; 0.03% Triton X-100) 50% bleach heptane methanol absolute ethanol (EtOH) xylene 5 mgs/ml proteinase K PBT + 2 mgs/ml glycine PBT + 50 mM EDTA nitroblue tetrazolium (NBT) (75 mgs/ml in DMF) X
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