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- 文献和实验
- 技术资料
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- 英文名：
Tetramethylrhodamine-4-iodoacetamide
- 库存：
100+
- 供应商：
上海维亦特生物
- CAS号：
114458-99-0
Tetramethylrhodamine-4-iodoacetamide
114458-99-0
C26H26I3N3O4
825.22
No Data Available
No Data Available
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文献和实验Tetramethylrhodamine-4-iodoacetamide
114458-99-0
C26H26I3N3O4
825.22
No Data Available
No Data Available
Labeling Gizzard Myosin with Tetramethylrhodamine Iodoacetamide (Primarily 17kDa Light Chain)
Day 1 Materials 1.50 mM KCl,0.2 mM EGTA,2 mM ATP,2 mM MgCl2,10 mM HEPES,pH 7.0.Need 500 ml. Procedure 1.Thaw appropriate volume of frozen gizzard myosin to obtain 10-20 mg. 2.Centrifuge in a SS34 rotor at 9,000 rpm,4℃ for 20 min. 3.Resuspend
Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
Materials 1.Drop frozen 2x cycled tubulin in a polymerization-promoting buffer.Stored at -80℃ 2.GTP,100 mM stock,titrate to pH ~7.Need 2 ml. 3.Waterbath,set at 37℃ 4.SS34 rotor,5 ml and 15 ml tubes and adaptors.Bring the rotor,adaptors,and tubes
Labeling Muscle Actin with N-(1-pyrene)iodoacetamide
Day 0 and 1 Materials 1.0.5 mM ATP,0.2 mM CaCl2,2 mM Tris-HCl,pH 8.0 at 4℃,250 ml for day 0. 2.0.5 mM ATP,2 mM MgCl2,100 mM boric acid,pH 8.3 at 4℃,10 ml. 3.N-(1-pyrene)iodoacetamide (Molecular probes P-29,m.w.385) 4.50Ti tubes,small vial
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