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- 英文名:
Ubiquinol-d3 [Labelled Mixture]
- 库存:
100+
- 供应商:
上海维亦特生物
- CAS号:
N/A
Ubiquinol-d3 [Labelled Mixture]
N/A
C₅₉H₈₉D₃O₄
868.37
No Data Available
No Data Available
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文献和实验Ubiquinol-d3 [Labelled Mixture]
N/A
C₅₉H₈₉D₃O₄
868.37
No Data Available
No Data Available
Fast and reliable mini-prep RNA extraction from Neurospora crassa
otherwise. - Pulverize the mycelium in a mortar with liquid nitrogen - Transfer the powder into a mixture of 0.75 ml lysis buffer (0.6 M NaCl, 10 mM EDTA, 100 mM Tris HCl, pH 8.0, 4 SDS) and 0.75 ml phenol (saturated with 0.1 M Tris HCl, pH 8.0) in a 2 ml
! Annealing[Annealing: Spontaneous alignment of two single DNA strands to form a double helix.]: The reaction mixture is cooled down.Primers anneal to the complementary regions in the DNA template [Template: A molecule that serves as the pattern
Generation of uni-directional nested deletions in plasmid DNA
. 2) Heat inactivate restriction enzymes at 85℃for 30 mins. 3) Phenol/chloroform, chloroform extract and alcohol precipitate digested DNA. 4) Redissolve DNA in 20μl 1x One Phor All buffer. 5) Add 20μl 2x ExoIII buffer and preincubate the mixture
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