2,8-Dichloro-4(3H)-quinazolino

Preparation of [3H]Phosphoinositol Standards and Conversion of [3H]Phosphoinositides to [3H]Phosphoinositols

Any chromatographic procedure for the separation of phosphoinositols will require a supply of standards, both for validation of the technique and identification of the products. Tritium-radiolabeled standards are commercially available in 1–

Proliferation Assay: [3H] Thymidine incorporation

cells for 2-4 hours then treat. 5) Six-twenty four hours before cells are to be harvested, add 1 µCi/ml [3H]-TdR to each well. Harvesting: 6) Wash cells 2 times with ice cold PBS. 7) Wash cells 2 times with 5% TCA.

3H-Thymidine Uptake by Cultured Cells

  Materials   Fibroblast cells in log phase growth Ca , Mg free-phosphate buffered saline (PBSA) 5% (w/v) Glutaraldehyde (GTA) 2% (w/v) Perchloric Acid (PCA) Subbed slides (coated with chrom alum gelatin