Mg(II) Porphine

Characterization of Angiotensin II Receptors

site. The K d for unlabeled Ang II is 3.934 nM, and the B max is 2.468 pmol/mg of protein. Data were analyzed using LIGAND. B, bound; F, free; T, unlabeled [Ang II] (M). View Image

Overexpression and Purification of Saccharomyces cerevisiae DNA Topoisomerase II from Yeast

have been immensely useful; 5 mg of wild-type or mutant topoisomerase II can generally be purified from 1 L of cells grown in selective media. This chapter describes methods for growing and inducing the yeast cells, and purifying the highly expressed type II DNA

PacBio RS II 的高通量测序应用

，DNA 聚合酶的合成速度会减慢，对应的信号会被检测出来。每种碱基修饰事件都会使聚合酶的「停顿模式」PacBio RS II 产生微小差异，最终反映到荧光脉冲信号的间隔上。除了甲基化修饰，还可以检测 5-hC、5-hmU、5-hU、1-mA、6-mA、8-oxoA、BPDE、6-mT、6-mG 等碱基修饰，甚至可以鉴别传统亚硫酸氢盐测序法无法区分的甲基化修饰和羟甲基化修饰。PacBio 平台可以在测序的同时即可检测表观遗传学修饰信息，只需对测序数据选择合适的软件即可分析碱基修饰信息。