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N-(1S,2S,3R,4S)-2,3,4-Tris(benzyloxy)-1-((benzyloxy)methyl)cyclohexanol (1S,2S,3R,4S,5S)-2,3,4-Tris(benzyloxy)-5-(((R)-1-(benzyloxy)-3-hydroxypropan-2-yl)amino)-1-((benzyloxy)methyl)cyclohexanol
N/A
C₈₀H₈₈N₂O₁₁
1253.56
No Data Available
Dichloromethane, Ethyl Acetate, Methanol
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文献和实验N-(1S,2S,3R,4S)-2,3,4-Tris(benzyloxy)-1-((benzyloxy)methyl)cyclohexanol (1S,2S,3R,4S,5S)-2,3,4-Tris(benzyloxy)-5-(((R)-1-(benzyloxy)-3-hydroxypropan-2-yl)amino)-1-((benzyloxy)methyl)cyclohexanol
N/A
C₈₀H₈₈N₂O₁₁
1253.56
No Data Available
Dichloromethane, Ethyl Acetate, Methanol
制法 A液:布氏杆菌肉汤 970mL 无水磷酸氢二钠 1.18g 无水磷酸二氢钾 0.23g 琼脂 2g 加热溶解,高压灭菌,121℃ 15min。加入B液10%硫酸亚铁(含7H2O),C液10%偏亚硫酸氢钠,D液10%丙酮酸钠。 上述B、C、D溶液均新鲜配制,用除菌滤膜过滤。将此除菌溶液各1mL混合后再入A液中,无菌调pH到7.3,无菌分装,每管3mL,塞紧备用
BACs, PACs, P1s and Plasmid Isolation Protocol (standard protocol)
1.5 ml centrifuge tube, apply 20-50 ul of Elution Buffer (10mM Tris-HCl, pH 8.5) or water onto the center of the membrane. Incubate 5 minutes at room temperature. 13. Centrifuge at maximum speed for 2 minutes to elute the DNA. 14. Store
BACs, PACs, P1s and Plasmid Isolation Protocol (For low copy number)
of Elution Buffer (10 mM Tris-HCl, pH 8.5), and incubating overnight at room temperature 12. Yield and quality of DNA: Determine the absorbance of an appropriate dilution of the sample at 260 nm and then at 280 nm. The DNA concentration is calculated
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