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18138-04-0
2,3-Diethyl-5-methylpyrazine
18138-04-0
C₉H₁₄N₂
150.22
Clear Colourless Oil
Chloroform, Methanol
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文献和实验2,3-Diethyl-5-methylpyrazine
18138-04-0
C₉H₁₄N₂
150.22
Clear Colourless Oil
Chloroform, Methanol
glassware and measuring spatulae; treat plastic ware (if not new and sterile) and miracloth with 0.1% diethyl pyrocarbonate (DEP) solution for at least 30 min. DEP-treat water, autoclave and use for making up solutions. Wear plastic gloves and use flamed
Oocyte isolation, maturation, enucleation and fractionation
container. Run Gel (look for ribosomal RNA bands). DEPCed H2O 100 µl of Diethyl Pyrocarbonate (in fridge “Sauron”) + 100 ml of dH20) into a 100 ml
Basic procedures for bacteria culture
and centrifuge to separate the phases. Discard the isobutanol (upper) phase, and extract once with water-saturated diethyl ether to remove residual isobutanol. The nucleic acid then may be ethanol precipitated as described above. E. Notes on phenol
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