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- 供应商:
上海维亦特生物
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Amylostatin XG Octamethoxybenzyl Ether
N/A
C₉₇H₁₀₉NO₂₁S
1656.96
Dark Red Solid
DMSO, Methanol
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文献和实验Amylostatin XG Octamethoxybenzyl Ether
N/A
C₉₇H₁₀₉NO₂₁S
1656.96
Dark Red Solid
DMSO, Methanol
Isolation of Microtubules (Bovine Brain)
( -aminoethyl Ether) N,N,N',N'-tetraacetic acid) 0.5 mM MgCl Adjust pH to 6.4 8 M Glycerol in MT buffer Virtis homogenizer (or equivalent) Refrigerated centrifuge GTP Bradford protein assay Procedure
Isolation of Microtubules (Bovine Brain)
)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis( -aminoethyl Ether) N,N,N',N'-tetraacetic acid) 0.5 mM MgCl Adjust pH to 6.4 8 M Glycerol in MT buffer Virtis homogenizer
Protein Syntheses in Cell Free Systems
.10 cells)rapidly in an ice bath.Collect the cells as a pellet,by centrifugation at 600 xg for 10 minutes at 4℃.Resuspend the cells in TBS buffer and wash them three times with cold TBS buffer.2.Suspend the final pellet in two volumes of TBS-M
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