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Tris-(2-methanethiosulfonylethyl)amine
N/A
C₉H₂₁NO₆S₆
431.66
White to Off-White Solid
Chloroform (Slightly, Heated), Ethyl Acetate (Slightly, Heated)
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文献和实验Tris-(2-methanethiosulfonylethyl)amine
N/A
C₉H₂₁NO₆S₆
431.66
White to Off-White Solid
Chloroform (Slightly, Heated), Ethyl Acetate (Slightly, Heated)
Preparation of probes for in situ hybridization
. Labeling is then conducted for 24-48 h in the dark, at room temperature, with occasional vortexing. NB: Any trace of free amine, such as Tris base, should be removed from the nucleic acid, as it will also react with the fluorophore.
Preparation of probes for in situ hybridization
temperature, with occasional vortexing. NB: Any trace of free amine, such as Tris base, should be removed from the nucleic acid, as it will also react with the fluorophore. Unreacted dye is then removed by either two rounds of ethanol
Fluorescein labeling of proteins
This method uses carboxyfluorescein succinimidyl ester (CFSE)rather than fluorescein isothiocyanate,resulting in more reliable labeling.Succinimidyl esters are excellent reagents for amine modification since the amide products formed are very stable
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