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Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) bis(hexafluorophosphate) complex
N/A
C₇₂H₄₈N₆Ru •2F₆P
N/A
Dark Orange to Very Dark Orange Solid
Acetonitrile (Slightly), Methanol (Slightly)
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文献和实验Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) bis(hexafluorophosphate) complex
N/A
C₇₂H₄₈N₆Ru •2F₆P
N/A
Dark Orange to Very Dark Orange Solid
Acetonitrile (Slightly), Methanol (Slightly)
-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166: 368-379. Reagents: Anode Buffer (+): 200 mM Tris pH 8.9 (can dilute from 10X stock) Cathode Buffer
by other suppliers can be 2-4 times higher than Kunitz units as measured at Worthington. At Worthington 0.005 Kunitz unit digest 1 ug of lambda DNA in 10 min at 37C in 50mM Tris, 1 mMMg++, pH 7.8 in a 50ul reaction. Correlation of digestion units with Kunitz units
Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
. No. 34080) For detection of interacting proteins by mass spectrometry we precipitate proteins before separation of complexes on NuPAGE 4-12% Bis-Tris Gradient gels (Invitrogen, Cat. No. NP0321) and Coomassie staining (Figure 1 ). We have a good
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