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9-cis-Retinol Acetate-d5 (>80%)
N/A
C₂₂H₂₇D₅O₂
333.52
Pale Yellow to Yellow Oil
Chloroform (Slightly), Methanol (Slightly)
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文献和实验9-cis-Retinol Acetate-d5 (>80%)
N/A
C₂₂H₂₇D₅O₂
333.52
Pale Yellow to Yellow Oil
Chloroform (Slightly), Methanol (Slightly)
Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
(see Hint #11). 8. Incubate at -80°C for between 30 min to overnight. 9. Centrifuge at 115,000 X g for 20 min at 4°C (35,000 rpm using an SW 50.1 rotor). 10. After centrifugation, remove the supernatant and vacuum dry
are precipitated with sodium acetate, and the lysate is cleared first by filtration of precipitate through cheesecloth and then by centrifugation. The DNA-containing supernatant is transferred to a new tube, and the plasmid or cosmid DNA is precipitated
Replication timing by density transfer
C]- acetate or glucose 0.01% (w/v) [15 N]- ammonium sulfate Amino acids/supplements (as required, added to the same concentration as in normal synthetic medium) --dissolved in "-N" medium and filtered
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