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(1S,5R,6S)-5-Isopropoxy-7-oxabicyclo[4.1.0]hept-3-ene-3-carboxylic Acid Ethyl Ester
N/A
C₁₂H₁₈O₄
226.27
White to Off-White Solid
Chloroform (Slightly), Methanol (Slightly)
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文献和实验(1S,5R,6S)-5-Isopropoxy-7-oxabicyclo[4.1.0]hept-3-ene-3-carboxylic Acid Ethyl Ester
N/A
C₁₂H₁₈O₄
226.27
White to Off-White Solid
Chloroform (Slightly), Methanol (Slightly)
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
The technique described here is a slight modification (March 1997) of methods presented in: Nagy A., J. Rossant. 1993. Production of completely ES cell-derived fetuses. In : Gene Targeting: A Practical Approach. (ed. A. Joyner). IRL Press at Oxford
in 37°C water bath for 10 minutes. 6) Centrifuge on setting 4 for 5 minutes. 7) Aspirate supernatant and flick pellet. In the next step, be careful to disperse the cells thoroughly in the fix. Add fresh fix (2 ml glacial acetic acid and 5 ml
An actively growing culture of cells is required, i e 2 - 3 d ES cell culture. The total number of cells needs to be between 106 - 107 cells. N B Read notes at end of method. 1) Harvest cells in the usual manner, into a conical
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