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isopropyl (4-(2-(5-Methylpyrazine-2-carboxamido)ethyl)phenyl)sulfonylcarbamate
N/A
C₁₈H₂₂N₄O₅S
406.46
White to Off-White Solid
Chloroform (Slightly), DMSO (Slightly), Methanol (Slightly)
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文献和实验isopropyl (4-(2-(5-Methylpyrazine-2-carboxamido)ethyl)phenyl)sulfonylcarbamate
N/A
C₁₈H₂₂N₄O₅S
406.46
White to Off-White Solid
Chloroform (Slightly), DMSO (Slightly), Methanol (Slightly)
for 10 minutes at room temperature.11. Transfer the upper aqueous layer to a new 1.5 mL tube and add equal volume of isopropyl alcohol.12. Mix well and incubate at -80oC for 12 minutes and spin at 14,000 rpm for 20 minutes at room temperature.13. Dispose
Assay for ß-galactosidase in E. Coli
, isopropyl-ß-thiogalactoside (IPTG), phenyl-ß-galactoside (PBG) and glucose (GLU). We will use a non-biological (artificial) substrate for the enzyme, Ortho-nitrophenyl-ß-galactoside (ONPG). In the presence of ß-galactosidase ONPG is converted
to dehydrate, clear and impregnate tissue samples. Fixation of tissue samples is achieved prior to processing in the microwave oven. processing schedules are developed using tissue thickness as a determining factor. Dehydration is accomplished using Ethyl
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