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- CAS号：
402616-26-6
(S) FTY720 Phosphate
402616-26-6
C₁₉H₃₄NO₅P
387.45
White to Pale Yellow Solid
Acidic Methanol (Slightly), DMSO (Very Slightly), Methanol (Very Slightly)
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文献和实验(S) FTY720 Phosphate
402616-26-6
C₁₉H₃₄NO₅P
387.45
White to Pale Yellow Solid
Acidic Methanol (Slightly), DMSO (Very Slightly), Methanol (Very Slightly)
Simplified Calcium Phosphate Coprecipitation
add 61 µL of 2M CaCl2 , plus 4 - 10 µg (1 - 2.5 µL) DNA, and q.s. to 0.5 mL by adding (438 µL) H2 O (or try TE pH8.0 ). Mix with a pipet and transfer dropwise to the 2x HEPES solution. Mix and then immediately sprinkle on top of the cultured cells
Cloning of small RNAs with 5’ phosphate and 3’ OH ends
. Back to top Procedure Purifying 20-30 Nucleotide lengths from 10µg of Total RNA (1 µg/µl). Instead of gel purification, mirVANA isolation can replace this step following manufacturer’s instructions. We have used from 1.5 to 5 µg
Caged Intracellular Messengers and the Inositol Phosphate Signaling Pathway
, derived from work in the author’s laboratory using caged inositol trisphosphate to study signaling in Xenopus oocytes.
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