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- CAS号：
1246816-60-3
8-Bromo-3-butyl-1-chloro-5,10-dihydroimidazo[1,5-b]isoquinolin-10(5H)-one
1246816-60-3
C₁₅H₁₄BrClN₂O
353.64
Off-White Solid
Chloroform (Slightly), Methanol (Slightly)
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文献和实验8-Bromo-3-butyl-1-chloro-5,10-dihydroimidazo[1,5-b]isoquinolin-10(5H)-one
1246816-60-3
C₁₅H₁₄BrClN₂O
353.64
Off-White Solid
Chloroform (Slightly), Methanol (Slightly)
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
immediately before using. Stocks X-Phos (100X):10mg/ml 5-bromo-4-chloro-3-indolyl-phosphate (also referred to as BCIP) in H20. Store in the dark as aliquots at -200 C. Can be frozen and thawed several times. NBT (50X):50 mg/ml nitro
Beta-Gal staining in primary neurons
2 Part b. X-gal (5-bromo-4-chloro-3-indolyl-beta-gal) 40 mg/ml in DMSO Heat part a & b to 37 C Add part b to part a to final 1 mg/ml X-gal. Add part b slowly while mixing, or crystals will form. Filter to remove crystals.
Agar Plates for Selection of Clones in Bacteria
C (can be stored in dark at 4C but -20 is usually as simple!) X-Gal: 20 mg/ml in Dimethylformamide (DMF) IPTG: 100 mM Ampicillin: 100 ug/ml (micrograms) Filter sterilize X-Gal is 5-bromo-4-chloro-3-indolyl-beta-D-galactoside. It is hydolysed
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