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- CAS号：
5070-13-3
Bis(4-nitrophenyl) Carbonate
5070-13-3
C₁₃H₈N₂O₇
304.21
White Solid
Chloroform, Ethyl Acetate
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文献和实验Bis(4-nitrophenyl) Carbonate
5070-13-3
C₁₃H₈N₂O₇
304.21
White Solid
Chloroform, Ethyl Acetate
β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer
to an equal volume of Assay 2X Buffer, which contains the substrate ONPG (o-nitrophenyl-beta-D-galactopyranoside). Samples are incubated for 30 minutes, during which time the Beta-Galactosidase hydrolyzes the colorless substrate to o-nitrophenyl
ELISA Procedure for Measuring Serum Antibody Titer
whole molecule/alkaline phosphatase conjugate p-Nitrophenyl phosphate Sodium carbonate buffer, 0.05 M pH 9.8, containing 1 mM magnesium chloride (MgCl2) Sodium hydroxide (NaOH), 2 M Procedure for Alkaline Phosphatase Detection1. Prepare a dilution of the goat
Assay for ß-galactosidase in E. Coli
, isopropyl-ß-thiogalactoside (IPTG), phenyl-ß-galactoside (PBG) and glucose (GLU). We will use a non-biological (artificial) substrate for the enzyme, Ortho-nitrophenyl-ß-galactoside (ONPG). In the presence of ß-galactosidase ONPG is converted
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