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- CAS号:
921757-00-8
4-Carbamoyl-1H-pyrrole-2-carboxylic Acid (10% inorganics)
921757-00-8
C₆H₆N₂O₃
154.12
Grey to Brown Solid
DMSO (Slightly)
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文献和实验4-Carbamoyl-1H-pyrrole-2-carboxylic Acid (10% inorganics)
921757-00-8
C₆H₆N₂O₃
154.12
Grey to Brown Solid
DMSO (Slightly)
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
Methods for the Detection of D-Amino-Acid Oxidase
on the activity of the samples. It was terminated by the addition of 1 ml of 10% trichloroacetic acid. In a blank, trichloroacetic acid was added to the reaction mixture before the enzyme reaction was started. The precipitate was removed by centrifugation (700 x g
核酸提取(The Extraction of Nucleic Acid)
f管(2ml,1.5ml,0.5ml),Eppendorff管架。 3.DNA提取的相关试剂: 1)蛋白酶K(10mg/ml),-20℃保存。 2)TE(pH 8.0):10mmol/L Tris.Cl(pH 8.0),1mmol/L EDTA(pH 8.0), 15磅高压灭菌后室温保存。 3)10% SDS:10g SDS溶于90ml 水中,加热68℃助溶,加几滴浓盐酸调节pH至7.2,加蒸馏水定容至100ml,室温保存。 4)3M NaAc: 800ml水中溶解408
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