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上海维亦特生物
- CAS号:
1639405-29-0
N,N'-Bis(2-aminoethyl)thiourea Dihydrochloride
1639405-29-0
C₅H₁₆Cl₂N₄S
235.18
Pale Orange to Pale Beige Solid
DMSO (Slightly), Methanol (Slightly, Heated), Water (Slightly)
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文献和实验N,N'-Bis(2-aminoethyl)thiourea Dihydrochloride
1639405-29-0
C₅H₁₆Cl₂N₄S
235.18
Pale Orange to Pale Beige Solid
DMSO (Slightly), Methanol (Slightly, Heated), Water (Slightly)
Isolation of Microtubules (Bovine Brain)
LEVEL II Materials Freshly removed bovine brain 2 Wire sieve (tea strainer) Microtubule buffer (MT buffer) 0.1 M MES (2-(N-Morphilino)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis
Isolation of Microtubules (Bovine Brain)
)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis( -aminoethyl Ether) N,N,N',N'-tetraacetic acid) 0.5 mM MgCl Adjust pH to 6.4 8 M Glycerol in MT buffer Virtis homogenizer
b-Gal Staining of Murine Embryos
PO4.H2O, 77ml of 0.2M Na2HPO4 and add 100ml DDW. the pH will be approx 7.3 0.5M EGTA in 0.1M Phosphate buffer, pH increase to 7. FW 380.4, so 19.02g for making 100ml of 0.5M EGTA. Ethylene Glycol-bis (b-aminoethyl ether)N,N,N',N'-tetraacetic acid
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